An Edible, Decellularized Plant Derived Cell Carrier for Lab Grown Meat

Rapidly expanding skeletal muscle satellite cells with cost-effective methods have been presented as a solution for meeting the growing global demand meat. A common strategy scaling cell proliferation employs microcarriers, small beads designed to support anchorage-dependent in suspension-style bioreactors. No carrier has yet marketed cultivation of lab-grown The objective this study was demonstrate rapid, food safe, decellularization procedure yield cell-free extracellular matrix scaffolds and evaluate them carriers lab grown Broccoli florets were soaked SDS, Tween-20, bleach 48 h. process confirmed via histology, which showed an absence nuclei, DNA quantification (0.0037 ± 0.00961 ?g DNA/mg tissue). Decellularized sorted by cross sectional area (7.07 1.74 mm2, 3.03 1.15 0.49 0.3 mm2) measured eccentricity (0.73 0.16). Density measurements decellularized (1.01 0.01 g/cm) comparable traditional microcarriers. Primary bovine inoculated into cultured within reactor containing carriers. Cell adhesion observed death limited 2.55 1.09%. These studies suggested that broccoli may serve adequate edible cells.